Human skeletal muscleGENE LPP1 Access # AB000888.1 Expression Strong detection Frequency 8/8 P-value 0.004 Function Dephosphorylates LPA, S1P, PA, ceramide-1-P GENE SUPPRESSED BY PHYSICAL INACTIVITYGENES 3-(Octyloxy)azetidine NOT AFFECTED BY PHYSICAL INACTIVITY LPP2 LPP3 AF047760.1 NM_003713 Not detected Strong detection 0/8 8/8 8/8 LPAP SGPP1 SGPL1 SPHK1 AGPAT1 AB031478.1 NM_030791 NM_003901.1 NM_021972.1 U56417 Moderate detection Moderate detection Not detected Not detected Strong detection 2/8 7/8 0/8 0/8 8/8 8/8 AGPAT2 AGPAT3 AGPAT4 LYPLA1 LYPLA2 EDG2 EDG3 EDG4 EDG7 EDG8 P2Y9 ROCK2 U56418 NM_020132 NM_020133.1 AF077198.1 NM_007260.1 45580697 NM_005226 NM_004720.3 NM_012152.1 AF317676 NM_005296.1 D87931 Moderate 1868-7083-5-S1-S3 detection Strong detection Not detected Strong detection Strong detection Moderate detection Not detected Not detected Not PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/21715270 detected Not detected Not detected Strong detection 8/8 8/8 0/8 8/8 8/8 4/8 0/8 0/8 0/8 0/8 0/8 7/8 0.47 0.0007 0.0002 0.068 0.024 0.90 0.19 0.0029 0.0056 0.028 0.002 0.25 0.0002 0.014 0.068 0.43 0.63 0.33 0.97 0.63 0.010 ER enzyme converting LPA to PA ER enzyme converting LPA to PA Converts LPA to PA Hydrolyzes lysophosphatidylcholine to LPA Hydrolyzes lysophosphatidylcholine to LPA LPA receptor LPA receptor LPA receptor LPA receptor S-1-P receptor LPA receptor, platelet LPA activated kinase Dephosphorylates LPA Dephosphorylates S1P Irreversible sphingosine 1-phosphate degradation Phosphorylates sphingosine ER enzyme converting LPA to PA Dephosphorylates LPA, S1P, PA, ceramide-1-P Dephosphorylates LPA, S1P, PA, ceramide-1-PAccess # is NCBI accession number. Frequency is the number of biopsy samples showing significant expression out of a total number of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/3081428 8 biopsies. P-value is the median p-value of a Wilcoxon test for all 8 human biopsy samples. The gene was bolded and the p-value was underlined if it met the FDR criteria for being significantly detected. Multiple probe sets are available for some genes and are presented in the Table. ER, endoplasmic reticulum; LPA, lysophosphatidic acid; S1P, sphingosine-1-phosphate; PA, phosphatidic acid LPA, lysophosphatidic acid; S1P, sphingosine-1-phosphate; PA, phosphatidic acid; AGPAT, 1-acylglycerol-3phosphate acyltransferase; EDG, endothelial differentiation gene; LPAP, LPA phosphatase; LPP, lipid phosphate phosphatase; LYPLA, lysophospholipase; ROCK2, Rho-associated, coiled-coil containing protein kinase 2; SGPL1, sphingosine-1-phosphate lyase 1; SGPP1, sphingosine-1-phosphatase; SPHK1, sphingosine kinase 1.LPP1 PCRPaired t-tests were used to compare 2-Chloro-3-methoxyaniline the PCR derived expression of LPP1 during sitting with normal standing/ambulatory activity in humans. A p < 0.05 was considered statistically significant. All data is presented as means ?SEM.ResultsExpression of hemostasis genes in skeletal muscleGenes were identified as being involved in hemostasis by searching PubMed and the publicly available Affymetrix NetAffyx website for key words related to hemostasis such as: coagulation, clotting, fibrinolysis, thrombosis, platelet aggregation, DVT, and hemostasis. First, we began this investigation in a large number of rats and the findings justified initiating a translational study in which human biopsies were obtained and extensively analyzed (44,792 probe sets). Genes involved in coagulation, anti-coagulation, andfibrinolysis are listed in Tables 1 (human) and 2 (rat). The absence and presence of expression for the set of genes related to LPP1 signaling are listed in Table 3. For some genes.